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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó; INIA Treinta y Tres. |
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Fecha : |
21/02/2014 |
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Actualizado : |
19/03/2021 |
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Tipo de producción científica : |
Artículos en Revistas Agropecuarias |
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Autor : |
BLANCO, P.H.; MOLINA, F.; PÉREZ DE VIDA, F.; ÁVILA, S.; LAVECCHIA, A.; MARCHESI, C.; DEAMBROSI, E.; MÉNDEZ, R.; SALDAIN, N.E.; ROEL, A.; ZORRILLA DE SAN MARTÍN, G.; ACEVEDO, A. |
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Afiliación : |
PEDRO HORACIO BLANCO BARRAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FEDERICO MOLINA CASELLA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FERNANDO BLAS PEREZ DE VIDA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIFLOR STELLA ÁVILA SILVA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANDRES PASCUAL LAVECCHIA GONZALEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CLAUDIA ELIZABETH MARCHESI GYERMAN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ENRIQUE GERMAN DEAMBROSI CHURRUT, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RAMÓN FELIPE MÉNDEZ LARROSA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; NÉSTOR ELIO SALDAIN CROCCE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ALVARO ROEL DELLAZOPPA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GONZALO ROBERTO ZORRILLA DE SAN MARTÍN PEREYRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANTONIO MARIA ACEVEDO VEGA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
INIA Olimar: características y comportamientos en la zafra 2003/2004. |
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Fecha de publicación : |
2004 |
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Fuente / Imprenta : |
Arroz, 2004, no. 38, p. 40-48. |
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Idioma : |
Español |
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Contenido : |
La nueva variedad tropical INIA Olimar se ha destacado a nivel experimental por su elevado potencial de rendimiento, estabilidad, precocidad y muy baja incidencia de granos yesados. En la Zafra pasada se sembró por primera vez un área significativa de esta variedad, constituida fundamentalmente por semilleros, alcanzando aproximadamente 3.000 hás. La disponibilidad de semilla producida permitiría un crecimiento importante de INIA Olimar, en caso de que el sector decida su adopción, por lo que es oportuno revisar sus características y el comportamiento en cultivos en la zafra 2003/04, la cual tuvo características climáticas particulares que permitieron que el país alcanzara un nuevo record de rendimiento. |
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Thesagro : |
ARROZ; ARROZ-GANADERÍA; INIA OLIMAR; PRODUCCIÓN DE ARROZ; PROGRAMAS DE INVESTIGACIÓN; SISTEMAS DE PRODUCCIÓN; VARIEDADES. |
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Asunto categoría : |
F01 Cultivo |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/11669/1/Arroz-2004-Blanco.pdf
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Marc : |
LEADER 01609naa a2200337 a 4500
001 1027837
005 2021-03-19
008 2004 bl uuuu u00u1 u #d
100 1 $aBLANCO, P.H.
245 $aINIA Olimar$bcaracterísticas y comportamientos en la zafra 2003/2004.
260 $c2004
520 $aLa nueva variedad tropical INIA Olimar se ha destacado a nivel experimental por su elevado potencial de rendimiento, estabilidad, precocidad y muy baja incidencia de granos yesados. En la Zafra pasada se sembró por primera vez un área significativa de esta variedad, constituida fundamentalmente por semilleros, alcanzando aproximadamente 3.000 hás. La disponibilidad de semilla producida permitiría un crecimiento importante de INIA Olimar, en caso de que el sector decida su adopción, por lo que es oportuno revisar sus características y el comportamiento en cultivos en la zafra 2003/04, la cual tuvo características climáticas particulares que permitieron que el país alcanzara un nuevo record de rendimiento.
650 $aARROZ
650 $aARROZ-GANADERÍA
650 $aINIA OLIMAR
650 $aPRODUCCIÓN DE ARROZ
650 $aPROGRAMAS DE INVESTIGACIÓN
650 $aSISTEMAS DE PRODUCCIÓN
650 $aVARIEDADES
700 1 $aMOLINA, F.
700 1 $aPÉREZ DE VIDA, F.
700 1 $aÁVILA, S.
700 1 $aLAVECCHIA, A.
700 1 $aMARCHESI, C.
700 1 $aDEAMBROSI, E.
700 1 $aMÉNDEZ, R.
700 1 $aSALDAIN, N.E.
700 1 $aROEL, A.
700 1 $aZORRILLA DE SAN MARTÍN, G.
700 1 $aACEVEDO, A.
773 $tArroz, 2004, no. 38, p. 40-48.
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Registro original : |
INIA Tacuarembó (TBO) |
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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
10/11/2023 |
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Actualizado : |
10/11/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
BORGES, M.A.; CURCIO, B.R.; GASTAL, G.D.A.; GHENO, L.; JUNIOR, A.S.V.; CORCINI, C.D.; NOGUEIRA, C.E.W.; AGUIAR, F.L.N.; GASTAL, E.L. |
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Afiliación : |
MORGANA A. BORGES, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; BRUNA R. CURCIO, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; GUSTAVO DESIRE ANTUNES GASTAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LUIZA GHENO, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; ANTONIO S. VARELA JUNIOR, Department of Comparative Animal Reproduction, Institute of Biological Sciences, Federal University of Rio Grande, RS, Rio Grande, Brazil; CARINE D. CORCINI, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; CARLOS E.W. NOGUEIRA, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; FRANCISCO L.N. AGUIAR, Department of Veterinary Medicine, Sousa Campus, Federal Institute of Education, Science and Technology of Paraiba, PB, Sousa, Brazil; EDUARDO L. GASTAL, Animal Science, School of Agricultural Sciences, Southern Illinois University, Carbondale, IL, United States. |
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Título : |
Ethanol, Carnoy, and paraformaldehyde as fixative solutions for histological evaluation of preantral follicles in equine ovarian tissue. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Reproductive Biology, December 2023, Volume 23, Issue 4, Article 100814. https://doi.org/10.1016/j.repbio.2023.100814 |
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ISSN : |
1642-431X |
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DOI : |
10.1016/j.repbio.2023.100814 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 28 February 2023; Received in revised form 24 September 2023; Accepted 27 September 2023; Available online 25 October 2023. -- Correspondence author: Gastal, E.L.; Animal Science, School of Agricultural Sciences, Southern Illinois University, 1205 Lincoln Drive, MC 4417, Carbondale, IL, United States; email:egastal@siu.edu -- FUNDING: This research was supported by grants from the Brazilian Criollo Horse Breeder Association (ABCCC) and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS; grant no. 21/2551-0002239-6). A.S. Varela Junior, C.D. Corcini, and B.R. Curcio had research productivity fellowships from the National Council for Scientific and Technological Development (CNPq). M.A. Borges and L. Gheno received DS and MS scholarships, respectively, from the Coordination for the Improvement of Higher Education Personnel (CAPES), Brazil. -- |
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Contenido : |
ABSTRACT.- The most adequate fixative solution for equine ovarian tissue is still to be determined as a tool to evaluate the improvement of methodological studies in assisted reproductive techniques and fertility preservation. This study aimed to evaluate a short-time ethanol 70% (ST-EtOH, 45 min) exposure as an alternative fixative compared with two classically fixatives [Carnoy's (CAR) solution and paraformaldehyde 4% (PFA)] at different fixation times (6 h, 12 h). The end points evaluated were morphology and classes of preantral follicles, follicular and stromal cell densities, and follicular and oocyte nuclear diameters in equine ovarian tissue. Ovaries (n = 6) from ovariectomized young mares were fragmented (3 × 3 × 1 mm; 20 fragments/ovary) and fixed in the tested treatments. Overall, a total of 11,661 preantral follicles were evaluated in 1444 histological slides. The ST-EtOH similarly preserved the preantral follicle morphometry and stromal cell density compared to the PFA fixative, regardless of the exposure time. Nonetheless, the CAR fixative solution had the greatest percentage of normal preantral follicles and the highest stromal cell density among all treatments. In conclusion, Carnoy's solution must be preferred compared with ST-EtOH and PFA fixatives for studies concerning the cellular morphology of equine ovarian tissue. Moreover, ST-EtOH fixative is a good alternative for equine ovarian tissue when a quick histological evaluation is required instead of more time-consuming and expensive techniques. Additional studies concerning the impact of different fixatives on the ultrastructure of cellular populations and their compatibility with IHC and molecular techniques in equine ovarian tissue are warranted. © 2023 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn MenosABSTRACT.- The most adequate fixative solution for equine ovarian tissue is still to be determined as a tool to evaluate the improvement of methodological studies in assisted reproductive techniques and fertility preservation. This study aimed to evaluate a short-time ethanol 70% (ST-EtOH, 45 min) exposure as an alternative fixative compared with two classically fixatives [Carnoy's (CAR) solution and paraformaldehyde 4% (PFA)] at different fixation times (6 h, 12 h). The end points evaluated were morphology and classes of preantral follicles, follicular and stromal cell densities, and follicular and oocyte nuclear diameters in equine ovarian tissue. Ovaries (n = 6) from ovariectomized young mares were fragmented (3 × 3 × 1 mm; 20 fragments/ovary) and fixed in the tested treatments. Overall, a total of 11,661 preantral follicles were evaluated in 1444 histological slides. The ST-EtOH similarly preserved the preantral follicle morphometry and stromal cell density compared to the PFA fixative, regardless of the exposure time. Nonetheless, the CAR fixative solution had the greatest percentage of normal preantral follicles and the highest stromal cell density among all treatments. In conclusion, Carnoy's solution must be preferred compared with ST-EtOH and PFA fixatives for studies concerning the cellular morphology of equine ovarian tissue. Moreover, ST-EtOH fixative is a good alternative for equine ovarian tissue when a quick histological evaluation is required instead of more ... Presentar Todo |
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Palabras claves : |
Fixative solution; Mare; Ovarian tissue quality; Preantral follicle morphometry. |
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Asunto categoría : |
L50 Fisiología y bioquímica animal |
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Marc : |
LEADER 03765naa a2200301 a 4500
001 1064365
005 2023-11-10
008 2023 bl uuuu u00u1 u #d
022 $a1642-431X
024 7 $a10.1016/j.repbio.2023.100814$2DOI
100 1 $aBORGES, M.A.
245 $aEthanol, Carnoy, and paraformaldehyde as fixative solutions for histological evaluation of preantral follicles in equine ovarian tissue.$h[electronic resource]
260 $c2023
500 $aArticle history: Received 28 February 2023; Received in revised form 24 September 2023; Accepted 27 September 2023; Available online 25 October 2023. -- Correspondence author: Gastal, E.L.; Animal Science, School of Agricultural Sciences, Southern Illinois University, 1205 Lincoln Drive, MC 4417, Carbondale, IL, United States; email:egastal@siu.edu -- FUNDING: This research was supported by grants from the Brazilian Criollo Horse Breeder Association (ABCCC) and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS; grant no. 21/2551-0002239-6). A.S. Varela Junior, C.D. Corcini, and B.R. Curcio had research productivity fellowships from the National Council for Scientific and Technological Development (CNPq). M.A. Borges and L. Gheno received DS and MS scholarships, respectively, from the Coordination for the Improvement of Higher Education Personnel (CAPES), Brazil. --
520 $aABSTRACT.- The most adequate fixative solution for equine ovarian tissue is still to be determined as a tool to evaluate the improvement of methodological studies in assisted reproductive techniques and fertility preservation. This study aimed to evaluate a short-time ethanol 70% (ST-EtOH, 45 min) exposure as an alternative fixative compared with two classically fixatives [Carnoy's (CAR) solution and paraformaldehyde 4% (PFA)] at different fixation times (6 h, 12 h). The end points evaluated were morphology and classes of preantral follicles, follicular and stromal cell densities, and follicular and oocyte nuclear diameters in equine ovarian tissue. Ovaries (n = 6) from ovariectomized young mares were fragmented (3 × 3 × 1 mm; 20 fragments/ovary) and fixed in the tested treatments. Overall, a total of 11,661 preantral follicles were evaluated in 1444 histological slides. The ST-EtOH similarly preserved the preantral follicle morphometry and stromal cell density compared to the PFA fixative, regardless of the exposure time. Nonetheless, the CAR fixative solution had the greatest percentage of normal preantral follicles and the highest stromal cell density among all treatments. In conclusion, Carnoy's solution must be preferred compared with ST-EtOH and PFA fixatives for studies concerning the cellular morphology of equine ovarian tissue. Moreover, ST-EtOH fixative is a good alternative for equine ovarian tissue when a quick histological evaluation is required instead of more time-consuming and expensive techniques. Additional studies concerning the impact of different fixatives on the ultrastructure of cellular populations and their compatibility with IHC and molecular techniques in equine ovarian tissue are warranted. © 2023 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn
653 $aFixative solution
653 $aMare
653 $aOvarian tissue quality
653 $aPreantral follicle morphometry
700 1 $aCURCIO, B.R.
700 1 $aGASTAL, G.D.A.
700 1 $aGHENO, L.
700 1 $aJUNIOR, A.S.V.
700 1 $aCORCINI, C.D.
700 1 $aNOGUEIRA, C.E.W.
700 1 $aAGUIAR, F.L.N.
700 1 $aGASTAL, E.L.
773 $tReproductive Biology, December 2023, Volume 23, Issue 4, Article 100814. https://doi.org/10.1016/j.repbio.2023.100814
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